Pharmacological studies of a lipase GA-56 produced by Pseudomonas sp. III. Subacute toxicity of GA-56.
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چکیده
منابع مشابه
Cloning, nucleotide sequencing, and expression in Escherichia coli of a lipase and its activator genes from Pseudomonas sp. KWI-56.
A lipase gene (lip) and its activator gene (act) on a 2.9 kb BglII-EcoRI fragment from Pseudomonas sp. KWI-56 were cloned in Escherichia coli using pUC19 as a vector plasmid. From the sequencing results, the open reading frames of the lip and the act were found to contain 1092 and 1032 nucleotides, respectively. The act existed downstream of the lip with the same orientation. When the lip was e...
متن کاملIn vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase.
The expression of lipase from Pseudomonas sp. strain KWI-56 (recently reclassified as Burkholderia cepacia) had been found to be dependent on an activator gene (act) downstream of its structural gene (lip). In this work, the mature lipase was synthesized in an enzymatically active form with a cell-free Escherichia coli S30 coupled transcription-translation system by expressing a recombinant lip...
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Polycrystalline Ni–Mn–Ga thin films have been deposited by the pulsed laser deposition ~PLD! technique, using slices of a Ni–Mn–Ga single crystal as targets and onto Si ~100! substrates at temperatures ranging from 673 K up to 973 K. Off-stoichiometry thin films were deposited at a base pressure of 1310 Torr or in a 5 mTorr Ar atmosphere. Samples deposited in vacuum and temperatures above 823 K...
متن کاملcharacterization of a lipase from a newly isolated pseudomonas sp.
background and objectives: lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. the aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme. materials and methods: a number of bacteria were isolated from sites related to the oil industries. an isolat...
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[This corrects the article on p. 383 in vol. 52.].
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ژورنال
عنوان ژورنال: Folia Pharmacologica Japonica
سال: 1974
ISSN: 0015-5691,1347-8397
DOI: 10.1254/fpj.70.71